Research Publications on "Sho-saiko-to" (1990-2001)

 

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Authors

  Inada Y.  Watanabe K.  Kamiyama M.  Kanemitsu T.  Clark WS.  Lange M.

Institution

  Department of Medicine, St Luke's-Roosevelt Hospital Center, College of

  Physicians and Surgeons of Columbia University, New York 10025, USA.

Title

  In vitro immunomodulatory effects of traditional Kampo medicine

  (sho-saiko-to: SST) on peripheral mononuclear cells in

  patients with AIDS.

Source

  Biomedicine & Pharmacotherapy.  44(1):17-9, 1990.

 

<77>

Authors

  Ono K.  Nakane H.  Fukushima M.  Chermann JC.  Barre-Sinoussi F.

Institution

  Laboratory of Viral Oncology, Aichi Cancer Center Research Institute, Nagoya,

  Japan.

Title

  Differential inhibition of the activities of reverse transcriptase and

  various cellular DNA polymerases by a traditional Kampo drug,

  sho-saiko-to.

Source

  Biomedicine & Pharmacotherapy.  44(1):13-6, 1990.

Abstract

  A traditional Kampo drug, Sho-saiko-to, composed of several

  herb extracts, differentially inhibited the activities of reverse

  transcriptase and human cellular DNA polymerase alpha and beta. Reverse

  transcriptases from murine leukemia virus and human immunodeficiency virus

  were inhibited by over 80% and 50%, respectively, in the presence of 100

  micrograms/ml Sho-saiko-to, whereas DNA polymerase alpha was

  much less sensitive to inhibition by this drug than were the reverse

  transcriptases. DNA polymerase gamma was not inhibited by this drug at

  concentrations of up to 500 micrograms/ml. Only DNA polymerase beta was

  moderately inhibited by Sho-saiko-to. Thus, it has been

  shown that the inhibition by Sho-saiko-to is relatively

  specific for reverse transcriptase and that the drug contains as yet

  unidentified inhibitory substance(s) for reverse transcriptase.

 

<78>

Authors

  Yonekura K.  Kawakita T.  Mitsuyama M.  Miura O.  Yumioka E.  Suzuki A.

  Nomoto K.

Institution

  Traditional Chinese Medicine Research Laboratories, Kanebo Co. Ltd., Osaka,

  Japan.

Title

  Induction of colony-stimulating factor(s) after administration of a

  traditional Chinese medicine, xiao-chai-hu-tang (Japanese name:

  shosaiko-to).

Source

  Immunopharmacology & Immunotoxicology.  12(4):647-67, 1990.

Abstract

  Colony stimulating factor (CSF)-rich serum was obtained from mice injected

  intraperitoneally (ip) with shosaiko-to, a traditional

  Chinese herbal medicine. Transfer of the CSF-rich serum into naive mice

  augmented the resistance against Listeria monocytogenes. A dose-dependent

  induction of CSF was observed in mice given shosaiko-to via

  intravenous route as well as via intraperitoneal route. Since the serum CSF

  induction was observed in both lipopolysaccharide (LPS)-responder C3H/He mice

  and LPS-non-responder C3H/HeJ mice, the effect of

  shosaiko-to seemed to be independent of possibly

  contaminating LPS. The level of serum CSF induced by

  shosaiko-to in athymic nude mice was similar to that in

  control euthymic mice, and the induction of CSF was completely blocked by the

  previous administration of carrageenan, a selective macrophage-blocker. In

  mice treated ip with shosaiko-to CSF activity was detected

  in the peritoneal cavity, the site of injection, and the time course was

  similar to that of serum CSF induction. In a bone marrow culture system, the

  composition of colonies formed by shosaiko-to-induced CSF

  was similar to that formed by standard GM-CSF. The CSF activity was scarcely

  affected by treatment of the sera with anti-M-CSF antibody. These results

  suggest that shosaiko-to augments the host defense by

  inducing mainly GM-CSF, and that CSF is produced by cells of macrophage

  lineage. In addition, it was shown that CSF could be induced even after oral

  administration of herbal medicines.

 

<79>

Authors

  Hiramatsu M.  Velasco RD.  Packer L.

Institution

  Department of Molecular and Cell Biology, University of California, Berkeley

  94720.

Title

  Vitamin E radical reaction with antioxidants in rat liver membranes.

Source

  Free Radical Biology & Medicine.  9(6):459-64, 1990.

Abstract

  The Japanese herbal medicine

  Sho-saiko-to-go-keishi-ka-shakuyaku-to (TJ-960) has been

  demonstrated to have an antioxidant action by quenching free radicals. The

  effects of TJ-960 on the tocopheroxy radicals generated by an arachidonic

  acid and lipoxygenase oxidation system were compared with those of the

  ascorbate and glutathione in vitamin E-enriched rat liver microsomes and

  submitochondrial membrane particles (SMP). Using electron spin resonance

  spectrometry, the disappearance of the tocopheroxy radicals after addition of

  glutathione and ascorbate was detected in microsomes and SMP, with ascorbate

  displaying a more potent action than glutathione. Addition of TJ-960

  demonstrated a similar effect on the tocopheroxy radicals in microsomes and

  SMP. In the presence of TJ-960, ascorbate, and glutathione, the loss of

  vitamin E in the vitamin E-enriched microsomes of rat liver undergoing

  oxidation was slowed down. In this paper, we introduced TJ-960 as another

  replenisher of vitamin E in membrane, increasing the membrane's resistance

  against oxidative damage.

 

<80>

Authors

  Buimovici-Klein E.  Mohan V.  Lange M.  Fenamore E.  Inada Y.  Cooper LZ.

Institution

  Department of Pediatrics, St. Luke's Roosevelt Hospital Center, New York, NY

  10019.

Title

  Inhibition of HIV replication in lymphocyte cultures of virus-positive

  subjects in the presence of sho-saiko-to, an oriental plant

  extract.

Source

  Antiviral Research.  14(4-5):279-86, 1990 Oct-Nov.

Abstract

  An oriental remedy, Sho-saiko-to (SST) consisting of a

  mixture of aqueous extracts from seven different plants and whose most active

  component is the chemically defined compound baicalein was tested for its

  ability to inhibit the production of the human immunodeficiency virus (HIV).

  The testing was done with cultures of human lymphocytes obtained from

  HIV-positive asymptomatic subjects and patients with ARC or AIDS. The

  replication of the virus was monitored by quantitative assay of the reverse

  transcriptase (RT) activity and of the synthesis of antigen p24. The

  lymphocyte cultures (LC) were maintained in the absence and in the presence

  of 25, 50 or 100 micrograms/ml of SST, and monitored for up to 5 weeks. The

  results showed that in LC from asymptomatic subjects RT activity and

  synthesis of p24 was completely inhibited by low concentrations of SST. High

  concentrations of SST inhibited virus replication in 80% of LC from ARC

  patients, but were completely ineffective in LC from AIDS patients. It was

  observed that the RT activity was more sensitive to inhibition by SST than

  the synthesis of p24, and that the antiviral effect was dependent on the

  virus load of the LC.

 

<81>

Authors

  Kawakita T.  Nakai S.  Kumazawa Y.  Miura O.  Yumioka E.  Nomoto K.

Institution

  Traditional Chinese Medicine Research Laboratories, Kanebo Co., Ltd, Osaka,

  Japan.

Title

  Induction of interferon after administration of a traditional Chinese

  medicine, xiao-chai-hu-tang (shosaiko-to).

Source

  International Journal of Immunopharmacology.  12(5):515-21, 1990.

Abstract

  We examined the ability of a traditional chinese herbal medicine,

  xiao-chai-hu-tang (Japanese name: shosaiko-to) to induce IFN

  in mice. A maximum activity (105 units/ml) of interferon (IFN) appeared in

  the serum of mice 16 h after intraperitoneal (i.p.) treatment with 250 mg/kg

  of shosaiko-to. Addition of polymyxin B did not abrogate the

  ability of shosaiko-to to induced serum IFN. The IFN was

  identified as IFN-alpha/beta by neutralizing test using anti-IFN alpha/beta

  antibodies. Pretreatment of mice with carrageenan suppressed the IFN

  induction by shosaiko-to, whereas the IFN induction by

  shosaiko-to was impaired neither in mice treated with

  anti-asialo-GM1 antibody nor in T-cell-deficient athymic nude mice. IFN was

  produced in vitro by spleen cells obtained from shosaiko-to

  treated mice. Moreover, spleen cells from untreated mice could also produce

  IFN when they were cultured with shosaiko-to. Additionally,

  serum IFN was also induced by the adoptive transfer of spleen cells from

  shosaiko-to treated mice to normal mice. On the other hand,

  peroral administration of shosaiko-to also induced

  IFN-alpha/beta in the serum. While IFN activity induced by i.p.

  administration of shosaiko-to declined after repeated

  treatments, the activity induced by its peroral administration did not

  decline during a long term treatment. These results showed that

  shosaiko-to is an IFN-alpha/beta inducer capable of repeated

  peroral administration.

 
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