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Research Publications on "Sho-saiko-to" (1990-2001)
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Is your "Sho-saiko-to" manufactured under the same quality standard as the ones approved by the Japanese Ministry of Health, Welfare, and Labor? Find out more... |
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<49> Authors
Liu L. Sakamoto S.
Nakayama T. Kudo H.
Suzuki S. Matsubara M.
Nagasawa
H. Institution
Medical Research Institute, Tokyo Medical and Dental University,
Japan. Title
Suppression by kampo medicine, sho-saiko-to, on papillomas
induced by 7,12-dimethylbenz(a)anthracene in mice. Source
American Journal of Chinese Medicine.
22(3-4):267-74, 1994. Abstract
Sho-saiko-to (SST) is a Japanese modified, traditional
Chinese herbal medicine (Kampo medicine) consisting of seven medical
plants.
We examined the effects of SST on formation and growth of squamous
cell
papillomas induced by 7,12-dimethylbenz(a)anthracene application in
mouse
skin. Chronic oral administration of SST reduced the incidence and
growth of
papillomas with the reduction of activities of
succinate-dehydrogenase and
thymidylate synthetase, which were evaluated as the cell viability
and DNA
synthesis via the de novo pathway, respectively. <50> Authors
Kawasaki A. Mizushima Y.
Kunitani H. Kitagawa M.
Kobayashi M. Institution
First Department of Internal Medicine, Toyama Medical and
Pharmaceutical
University, Japan. Title
A useful diagnostic method for drug-induced pneumonitis: a case
report. Source
American Journal of Chinese Medicine.
22(3-4):329-36, 1994. Abstract
A 51 year-old male was admitted to our hospital with chief complaints
of
fever, dry cough and dyspnea. Chest X-ray films and his history of
taking
Chinese medicine for liver dysfunction were suggestive of
drug-induced
pneumonitis. Lymphocyte stimulation test (LST) to causative Chinese
medical
drugs of Sho-saiko-to and Dai-saiko-to was negative with
peripheral blood lymphocytes (PBL), but was positive with lymphocytes
from
bronchoalveolar lavage fluid (BALF). In vivo challenge test for
Sho-saiko-to was positive. The LST with BALF-lymphocytes
proved to be very useful in making a diagnosis of drug-induced
pneumonitis. <51> Authors
Yoshida K. Mizukawa H.
Honmura A. Uchiyama Y.
Nakajima S. Haruki E. Institution
Kanagawa Rehabilitation institute, Japan. Title
The effect of sho-saiko-to on concentration of vitamin E in
serum and on granuloma formation in carrageenin cotton pellet-induced
granuloma rats. Source
American Journal of Chinese Medicine.
22(2):183-9, 1994. Abstract
The effect of Sho-saiko-to on the concentration of vitamin E
in serum and on the granuloma formation in Carrageenin cotton
pellet-induced
rats was investigated. As a result, in the granuloma rats of
Sho-saiko-to group, a significantly improved inhibitory
effect on granuloma formation and a higher concentration of vitamin E
in
serum, cholesterol and phospholipid were observed compared to the
control
group. Despite this lipid-increasing action by Sho-saiko-to,
the concentration of serum lipid peroxide was significantly lower
than in the
control group. Furthermore, a significant negative correlation
between the
concentration of vitamin E and granuloma weight was observed. These
results
suggest that vitamin E plays an important role in promoting the
anti-inflammatory effect of Sho-saiko-to. <52> Authors
Motoo Y. Sawabu N. Institution
Department of Internal Medicine, Kanazawa University, Japan. Title
Antitumor effects of saikosaponins, baicalin and baicalein on human
hepatoma
cell lines. Source
Cancer Letters. 86(1):91-5, 1994 Oct 28. Abstract
Antitumor effects of nine components of a herbal medicine,
'Sho-saiko-to', were investigated on human hepatoma cell
lines (PLC/PRF/5, Hep-G2), human liver cells (Chang) and a human
pancreatic
cancer cell line (BxPC-3). The concentration of each component
required for
50% inhibition of cell growth of PLC/PRF/5 cells was as follows:
saikosaponin-d, baicalin, 20 micrograms/ml; saikosaponin-a, baicalein,
50
micrograms/ml; saikosaponin-b2, -c, ginsenoside-Rb1, -Rg1,
glycyrrhizin, >
1000 micrograms/ml. Saikosaponin-a in 50-micrograms/ml quantities
inhibited
the cell growth and DNA synthesis of all the cell lines tested. These
results
indicate that 'Sho-saiko-to' includes potent antitumor
components such as saikosaponin-a, -d, baicalin against human
hepatoma cells
as well as other human cell lines. <53> Authors
Sakamoto S. Furuichi R.
Matsuda M. Kudo H.
Suzuki S. Sugiura Y.
Kuwa K.
Tajima M. Matsubara M.
Namiki H. et al. Institution
Medical Research Institute, Tokyo Medical and Dental University,
Japan. Title
Effects of Chinese herbal medicines on DNA-synthesizing enzyme
activities in
mammary tumors of mice. Source
American Journal of Chinese Medicine.
22(1):43-50, 1994. Abstract
Sho-saiko-to (SST) and Juzen-taiho-to (JTT), Japanese
modified Chinese herbal prescriptions, suppressed the activities of
thymidylate synthetase and thymidine kinase involved in de novo and
salvage
pathways for pyrimidine nucleotide synthesis, respectively, in
mammary tumors
of SHN mice with the reduction of serum prolactin level. These
results
indicate that SST and JTT may have the antitumor effects on mammary
tumors. <54> Authors
Kawakatsu T. Nomura S. Kido
H. Yamaguchi K. Fukuroi T. Suzuki
M. Yanabu
M. Kokawa T.
Yasunaga K. Institution
First Department of Internal Medicine, Kansai Medical University,
Osaka,
Japan. Title
Effect of three Japanese kampo medicines on platelet activation by
monoclonal
anti-platelet membrane glycoprotein antibodies. Source
American Journal of Chinese Medicine.
22(1):71-6, 1994. Abstract
We studied the effect of three Japanese kampo medicines on platelet
activation by an anti-CD9 monoclonal antibody (NNKY1-19) and an
anti-human Fc
gamma receptor II monoclonal antibody (NNKY3-2).
Sho-saiko-to (TJ-9) and Sairei-to (TJ-114) partially
suppressed platelet aggregation induced by NNKY1-19, while
Juzen-taiho-to
(TJ-48) suppressed aggregation induced by NNKY3-2. TJ-9 and TJ-114
also
suppressed collagen-induced aggregation, but TJ-48 did not. Flow
cytometry
showed that the three medicines did not affect antibody binding to
the
platelets. Thus, all three kampo medicines suppressed platelet
activation by
anti-platelet glycoprotein antibodies without inhibiting antibody
binding. <55> Authors
Sakaguchi S. Tsutsumi E. Yokota
K. Institution
First Department of Hygienic Chemistry, Tohoku College of Pharmacy,
Sendai,
Japan. Title
Defense effects of a traditional Chinese medicine
(sho-saiko-to) against metabolic disorders during
endotoxemia; approached from the behavior of the calcium ion. Source
Biological & Pharmaceutical Bulletin.
17(2):232-6, 1994 Feb. Abstract
The present study was carried out as an approach from intracellular
Ca2+ to
clarify the preventive effects of a traditional Chinese medicine,
Sho-saiko-to (Kampo prescription, TJ-9), against various
metabolic disorders during endotoxemia. In this experiment, we
estimated the
cytosolic-free Ca2+ concentration ([Ca2+]i) in liver single cells
using a
photonic microscope system. The [Ca2+]i in a single liver cell of
endotoxin
(6 mg/kg, i.p.)-injected mice was greater at 18 h post-intoxication
than that
in the control, whereas the administration of endotoxinin to TJ-9
(500
mg/kg/d, p.o.)-pretreated mice resulted in a markedly lower level of
[Ca2+]i
than that in endotoxin-treated mice. In the mice pretreated with
TJ-9, the
CA(2+)-ATPase activity in liver plasma membrane 18 h after endotoxin
injection was markedly increased as compared to that in the endotoxin-treated
mice. By contrast, the Ca(2+)-ATPase activity in liver mitochondria
was lower
in endotoxemic mice pretreated with TJ-9 than in mice given endotoxin
alone.
State 3 respiration and the respiratory control index (RCI), which
are the
parameters of mitochondrial function, were 41% and 35% lower,
respectively,
in the liver of mice given endotoxin than those levels in the
control.
However, the levels of state 3 and RCI in endotoxin-TJ-9-treated mice
were
markedly increased as compared to those of the endotoxin-treated
mice. These
findings suggest the protective effect of TJ-9 in the damage of liver
mitochondrial function in endotoxin-poisoned mice.(ABSTRACT TRUNCATED
AT 250
WORDS) <56> Authors
Hasegawa T. Yamaki K.
Nadai M. Muraoka I.
Wang L. Takagi K.
Nabeshima
T. Institution
Department of Hospital Pharmacy, Nagoya University School of
Medicine, Japan. Title
Lack of effect of Chinese medicines on bioavailability of ofloxacin
in
healthy volunteers. Source
International Journal of Clinical Pharmacology & Therapeutics.
32(2):57-61,
1994 Feb. Abstract
Recently, Chinese medicines have become available as OTC drugs and
are
frequently prescribed with Western medicine for the treatment of
various
chronic diseases. In this study, the effect of the Chinese medicines
Sho-saiko-to (TJ-9), Rikkunshi-to (TJ-43) and Sairei-to
(TJ-114) on the bioavailability of ofloxacin (OFLX) was investigated
in seven
volunteers in an open, random crossover fashion. Subjects received a
single
oral dose of OFLX (200 mg) alone and with coadministrations of each
Chinese
medicine, at one-week intervals. Plasma and urine samples were
analyzed by
high-performance liquid chromatography. No significant differences in
any
estimated bioavailability parameters of OFLX were observed between
the two
phases. The urinary recovery of OFLX excreted within 24 h after the
administration of OFLX alone, 80.6 +/- 3.9% (mean +/- SEM), was not
significantly different from those after the coadministrations of the
Chinese
medicines (79.7 +/- 5.1% for TJ-9, 76.8 +/- 2.3% for TJ-43 and 80.3
+/- 5.3%
for TJ-114), suggesting that there was no difference in the systemic
availability of the four doses. These findings indicate that the
Chinese
medicines studied have no significant effect on the rate and extent
of
bioavailability of OFLX. <57> Authors
Kaneko M. Kawakita T.
Tauchi Y. Saito Y.
Suzuki A. Nomoto K. Institution
Traditional Chinese Medicine Research Laboratories, Kanebo Co., Ltd,
Osaka. Title
Augmentation of NK activity after oral administration of a
traditional
Chinese medicine, xiao-chai-hu-tang (shosaiko-to). Source
Immunopharmacology & Immunotoxicology.
16(1):41-53, 1994 Feb. Abstract
We have shown that a traditional Chinese medicine, Xiao-chai-hu-tang
(Japanese name: Shosaiko-to) augments natural killer (NK)1
activity in mice. The maximum augmentation of NK activity in the
peripheral
blood and liver was observed at 12 hr after administration of
Shosaiko-to. NK activity was augmented by
Shosaiko-to dose-dependently. The augmentation became
significantly positive at a dose of 500 mg/kg, and the maximum effect
was
observed at a dose of 1000 mg/kg. The augmentation of NK activity
appeared at
first in the liver from 6 hr after administration of
Shosaiko-to and became detectable later in the peripheral
blood from 12 hr after the administration. Activation of NK cells by
Shosaiko-to may occur in the liver and subsequently the
activated NK cells may be supplied to the peripheral blood. Changes
in
percentages of cell surface markers (asialo GM1, CD3, CD4, CD8) after
Shosaiko-to treatment were hardly detected, but augmentation
of NK activity induced by Shosaiko-to was abrogated by
anti-asialo GM1 antibody treatment before the cytotoxicity assay. In
addition, cytotoxic activity to P-815 target cells was not detected
in
Shosaiko-to treated mice. Augmentation of NK activity by
Shosaiko-to is probably mediated by functional activation of
classical NK cells of asialo GM1+ phenotype. These results suggest
that
augmentation of NK activity in the liver is one of mechanisms
involved in
clinical efficacy of Shosaiko-to in patients with virus
chronic hepatitis. <58> Authors
Yano H. Mizoguchi A.
Fukuda K. Haramaki M.
Ogasawara S. Momosaki S.
Kojiro M. Institution
First Department of Pathology, Kurume University School of Medicine,
Japan. Title
The herbal medicine sho-saiko-to inhibits proliferation of
cancer cell lines by inducing apoptosis and arrest at the G0/G1
phase. Source
Cancer Research. 54(2):448-54, 1994 Jan 15. Abstract
Water-soluble ingredients of the herbal medicine
sho-saiko-to dose-dependently inhibited the proliferation of
a human hepatocellular carcinoma cell line (KIM-1) and a
cholangiocarcinoma
cell line (KMC-1). Fifty % effective doses on day 3 of exposure to
sho-saiko-to were 353.5 +/- 32.4 micrograms/ml for KIM-1 and
236.3 +/- 26.5 micrograms/ml for KMC-1. However, almost no
suppressive
effects were detected in normal human peripheral blood lymphocytes or
normal
rat hepatocytes. Sho-saiko-to suppressed the proliferation
of the carcinoma cell lines significantly more strongly than did each
of its
major ingredients, i.e., saikosaponin a, c, and d, ginsenoside Rb1
and Rg1,
glycyrrhizin, baicalin, baicalein, and wogonin, or another herbal
medicine,
juzen-taiho-to (P < 0.05 or 0.005). Because such ingredients are
barely
soluble in water, there could be synergistic or additive effects of
the
ingredients in sho-saiko-to. Morphological, DNA, and cell
cycle analyses revealed two possible modes of action of sho-saiko-to to suppress the proliferation of carcinoma
cells; (a) it induces apoptosis in the early period of exposure and
(b) it
induces arrest at the G0/G1 phase in the late period of exposure. |
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